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KMID : 0923620080080010013
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Immune Network
2008 Volume.8 No. 1 p.13 ~ p.20
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Coptis chinensis Extract Inhibits the Production of Inflammatory Mediators and Delayed Type Hypersensitivity in Mice
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Lee Yeon-Ah
Hong Seung-Jae
Lee Snag-Hoon
Kim Kyung-Soo
Jung Ki-Won
Han Chung-Soo
Yoo Myung-Chul
Yang Hyung-In
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Abstract
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Background:Coptis chinensis rhizome has been used as a medicinal herb in traditional Oriental medicine. We investigated the effects of Coptis chinensis extract on inflammatory mediators and delayed type hypersensitivity in mice.
Methods: The inhibitory effect of ethanolic extract of Coptis chinensis (CCE) on cell proliferation was evaluated using MTS assay. The lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the Con A-activated mouse splenocytes were cultured with various concentrations of CCE. Total nitric oxide (NO) production was determined by Griess reaction. The amounts of secreted prostaglandine E2 (PGE2), interleukin (IL)-2 and IFN-? were measured by ELISA. To investigate the in vivo anti-inflammatory effect of CCE, oxazolone-induced delayed type hypersensitivity (DTH) model was used.
Results: The CCE at 100 ?g/ml significantly blocked the LPS-induced production of pro-inflammatory mediators (NO and PGE) in RAW264.7 macrophages. Also, it significantly inhibited cell proliferation and cytokine (IL-2 and IFN-?) production in splenocytes. Furthermore, when splenocytes from CCE fed mice (200 mg/kg for 2 weeks) were activated with Con A, cell proliferation and cytokine production were significantly inhibited. In addition, CCE decreased in vivo inflammation in oxazolone-induced DTH model mice.
Conclusion: We suggest that Coptis chinensis can be used as an anti-inflammatory drug by exerting an inhibitory effect in inflammatory mediator- and cell-mediated inflammation. [Immune Network 2008;8(1):13-20]
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KEYWORD
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Coptis chinensis, inflammatory cytokine, inflammatory mediator, delayed type hypersensitivity (DTH)
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